Rabbit Anti-PCNA Quantity size : 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent ) Background: Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure. Specificity : ·anti-PCNA is a rabbit polyclonal antibody unconjugated ·specific for PCNA of human, mouse, rat, rabbit, horse, bovine, dog, chicken and sheep ·epitope mapping in the middle of PCNA of human, mouse, rat, rabbit, horse, bovine, dog, chicken and sheep origin ·use for western blotting, elisa, immunoprecipitation and immunohistochemistry · affinity chromatography purification, purity :>95% · Isotype: IgG · mol wt: 29kDa Application: ·Western blotting 1:100-500 ·Immunohistochemistry 1:100-500 ·ELISA 1:500-1000 · Optimal working dilutions must be determined by the end user. Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
